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MAGEA10 Polyclonal Antibody, FITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Sheep
Overview
Catalog # bs-6816R-FITC-100uL
Product Name MAGEA10 Polyclonal Antibody, FITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Sheep
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MAGEA10
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4109
Subcellular location Nucleus
Synonyms Cancer/testis antigen 1.10; CT1.10; MAGE 10 antigen; MAGE10; Melanoma antigen family A 10; Melanoma associated antigen 10; MAGAA_HUMAN.
Background This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream melanoma antigen family A, 5 (MAGEA5) gene.[provided by RefSeq, Oct 2011].
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MAGEA10 Polyclonal Antibody, FITC Conjugated (bs-6816R-FITC) validated for, and at what dilution?

bs-6816R-FITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MAGEA10 Polyclonal Antibody, FITC Conjugated (bs-6816R-FITC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep.

How should bs-6816R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MAGEA10 Polyclonal Antibody, FITC Conjugated (bs-6816R-FITC)?

KLH conjugated synthetic peptide derived from human MAGEA10

What are the excitation and emission wavelengths of the FITC conjugate (bs-6816R-FITC)?

FITC on bs-6816R-FITC: excitation / emission 494nm/518nm.