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MAGEA11 Polyclonal Antibody

Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti MAGEA11 Polyclonal Antibody, Unconjugated (bs-6817R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 48kD. Observed band size: 48kD.
Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti MAGEA11 Polyclonal Antibody, Unconjugated (bs-6817R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 48kD. Observed band size: 48kD.
Lane 1: Human A431 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U-2 OS cell lysates probed with MAGEA11 Polyclonal Antibody, Unconjugated (bs-6817R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Lane 1: Human A431 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human U-2 OS cell lysates probed with MAGEA11 Polyclonal Antibody, Unconjugated (bs-6817R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MAGEA11) Polyclonal Antibody, Unconjugated (bs-6817R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (MAGEA11) Polyclonal Antibody, Unconjugated (bs-6817R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bs-6817R
Product Name MAGEA11 Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MAGEA11
Immunogen Range 12-120/429
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4110
Swiss Prot P43364
Subcellular location Cytoplasm, Nucleus
Synonyms CT1.11; MAGE11; MAGE-11; MAGEA-11; Melanoma-associated antigen 11; Cancer/testis antigen 1.11; MAGE-11 antigen; MAGEA11
Background Acts as androgen receptor coregulator that increases androgen receptor activity by modulating the receptors interdomain interaction. May play a role in embryonal development and tumor transformation or aspects of tumor progression.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MAGEA11 Polyclonal Antibody (bs-6817R) validated for, and at what dilution?

bs-6817R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MAGEA11 Polyclonal Antibody (bs-6817R) react with?

Tested reactivity: Human.

How should bs-6817R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MAGEA11 Polyclonal Antibody (bs-6817R)?

KLH conjugated synthetic peptide derived from human MAGEA11 Immunogen range: 12-120/429.

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