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MAGEB6 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bs-6825R-Cy3
Product Name MAGEB6 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MAGEB6
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 158809
Swiss Prot Q8N7X4
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms CT3.4; MAGE-B6; MAGEB6A; Melanoma-associated antigen B6; Cancer/testis antigen 3.4; MAGE-B6 antigen; MAGEB6
Background This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. This gene is expressed in testis, and in a significant fraction of tumors of various histological types. The MAGEB genes are clustered on chromosome Xp22-p21. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MAGEB6 Polyclonal Antibody, Cy3 Conjugated (bs-6825R-Cy3) validated for, and at what dilution?

bs-6825R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MAGEB6 Polyclonal Antibody, Cy3 Conjugated (bs-6825R-Cy3) react with?

Tested reactivity: Human.

How should bs-6825R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MAGEB6 Polyclonal Antibody, Cy3 Conjugated (bs-6825R-Cy3)?

KLH conjugated synthetic peptide derived from human MAGEB6

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6825R-Cy3)?

Cy3 on bs-6825R-Cy3: excitation / emission 512,550nm/570,615nm.