Nucleostemin Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6846R-RBITC |
| Product Name | Nucleostemin Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Nucleostemin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26354 |
| Subcellular location | Nucleus |
| Synonyms | E2 induced gene 3 protein; E2IG3; Estradiol induced nucleotide binding protein; GNL3; Guanine nucleotide binding protein like 3 nucleolar; Guanine nucleotide binding protein like 3; MGC800; NNP47; Novel nucleolar protein 47; NS; Nucleolar GTP binding protein 3; Nucleostemin; Putative nucleotide binding protein estradiol induced; C77032; GNL3_HUMAN. |
| Background | Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell cycle progression in stem cells and cancer cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Nucleostemin Polyclonal Antibody, RBITC Conjugated (bs-6846R-RBITC) validated for, and at what dilution?
bs-6846R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nucleostemin Polyclonal Antibody, RBITC Conjugated (bs-6846R-RBITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig.
How should bs-6846R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nucleostemin Polyclonal Antibody, RBITC Conjugated (bs-6846R-RBITC)?
KLH conjugated synthetic peptide derived from human Nucleostemin
What are the excitation and emission wavelengths of the RBITC conjugate (bs-6846R-RBITC)?
RBITC on bs-6846R-RBITC: excitation / emission 540nm/625nm.