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Nucleostemin Polyclonal Antibody, PE-Cy5.5 Conjugated

Applications

  • WB

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
Overview
Catalog # bs-6846R-PE-Cy5.5
Product Name Nucleostemin Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
WB
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig
Specifications
Conjugation PE-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Nucleostemin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 26354
Subcellular location Nucleus
Synonyms E2 induced gene 3 protein; E2IG3; Estradiol induced nucleotide binding protein; GNL3; Guanine nucleotide binding protein like 3 nucleolar; Guanine nucleotide binding protein like 3; MGC800; NNP47; Novel nucleolar protein 47; NS; Nucleolar GTP binding protein 3; Nucleostemin; Putative nucleotide binding protein estradiol induced; C77032; GNL3_HUMAN.
Background Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell cycle progression in stem cells and cancer cells.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Nucleostemin Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-6846R-PE-CY5-5) validated for, and at what dilution?

BS-6846R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Nucleostemin Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-6846R-PE-CY5-5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig.

How should BS-6846R-PE-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Nucleostemin Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-6846R-PE-CY5-5)?

KLH conjugated synthetic peptide derived from human Nucleostemin

What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-6846R-PE-CY5-5)?

PE-Cy5.5 on BS-6846R-PE-CY5-5: excitation / emission 488nm/694nm.