FSCN1 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6848R-BF488-100uL |
| Product Name | FSCN1 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Fascin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6624 |
| Subcellular location | Cytoplasm |
| Synonyms | 55 kDa actin bundling protein; 55 kDa actin-bundling protein; Actin bundling protein; FAN 1; Fscin1; FAN1; Fascin 1; Fascin; Fascin homolog 1 actin bundling protein Strongylocentrotus purpuratus; Fascin homolog 1; Fascin1; FLJ38511; FSCN 1; FSCN1; FSCN1_HUMAN; HSN; p55; Singed Drosophila like sea urchin fascin homolog like; Singed drosophila homolog like; Singed like fascin homolog sea urchin Drosophila; Singed like fascin homolog sea urchin; Singed like protein; Singed-like protein; SNL; Strongylocentrotus purpuratus. |
| Background | Fascin is a highly conserved actin-bundling protein. Fascin, encoded by the human homolog for sn (hsn) gene, has been localized to microspikes and stress fibers of cultured cells where it is thought to be involved in the formation of microfilament bundles. It is expressed predominantly in dendritic cells. Lymphoid cells, myeloid cells and plasma cells are negative. However, Reed Sternberg cells in Hodgkin?s lymphoma are positive for fascin staining. Epstein-Barr virus may induce expression of fascin in B cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FSCN1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-6848R-BF488) validated for, and at what dilution?
bs-6848R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FSCN1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-6848R-BF488) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog.
How should bs-6848R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FSCN1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-6848R-BF488)?
KLH conjugated synthetic peptide derived from human Fascin
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-6848R-BF488)?
AbBy Fluor-488 on bs-6848R-BF488: excitation / emission 499nm/519nm.
