NALP4 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6855R-BF350-100uL |
| Product Name | NALP4 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody will detect multiple forms of NALP4, including the A, C and E Mouse variants. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NALP4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 147945 |
| Swiss Prot | Q96MN2 |
| Subcellular location | Cytoplasm, Secreted, Extracellular matrix |
| Synonyms | NACHT, LRR and PYD domains-containing protein 4; Cancer/testis antigen 58; CT58; PAAD and NACHT-containing protein 2; PAN2; PYRIN and NACHT-containing protein 2; PYRIN-containing APAF1-like protein 4; PYPAF4; Ribonuclease inhibitor 2; NLRP4; NALP4; RNH2 |
| Background | May be involved in inflammation and recognition of cytosolic pathogen-associated molecular patterns (PAMPs) not intercepted by membrane-bound receptors. Acts as a negative regulator of the type I interferon signaling pathway by serving as an adapter to promote DTX4-mediated ubiquitination of activated TBK1, and its subsequent degradation. Suppresses NF-kappaB induction by the cytokines TNFA and IL1B, suggesting that it operates at a point of convergence in these two cytokine signaling pathways. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NALP4 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-6855R-BF350) validated for, and at what dilution?
bs-6855R-BF350 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NALP4 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-6855R-BF350) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Horse.
How should bs-6855R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NALP4 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-6855R-BF350)?
KLH conjugated synthetic peptide derived from human NALP4
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-6855R-BF350)?
AbBy Fluor-350 on bs-6855R-BF350: excitation / emission 346nm/442nm.