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BRF2 Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Pig
Overview
Catalog # bs-6882R-APC-Cy7-100uL
Product Name BRF2 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Pig
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BRF2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 678
Subcellular location Nucleus
Synonyms ZFP36L2; BRF2; Butyrate response factor 2; EGF response factor 2; ERF 2; ERF2; Protein TIS11D; RNF162C; zinc finger protein 36, C3H type like 2; BRF2_HUMAN.
Background TIS11D is a member of the TIS11 family of early response genes. Family members are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. The encoded protein contains a distinguishing putative zinc finger domain with a repeating cys-his motif. This putative nuclear transcription factor most likely functions in regulating the response to growth factors.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is BRF2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-6882R-APC-Cy7) validated for, and at what dilution?

bs-6882R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BRF2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-6882R-APC-Cy7) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Pig.

How should bs-6882R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BRF2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-6882R-APC-Cy7)?

KLH conjugated synthetic peptide derived from human BRF2

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-6882R-APC-Cy7)?

APC-Cy7 on bs-6882R-APC-Cy7: excitation / emission 650nm/780nm.

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