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KLLN Polyclonal Antibody

Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with KLLN Polyclonal Antibody(bs-6893R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with KLLN Polyclonal Antibody(bs-6893R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Bioss unconjugated antibody vial

Applications

  • ELISA
  • FCM
  • IHC-P

Reactivity

  • Human
Overview
Catalog # bs-6893R
Product Name KLLN Polyclonal Antibody
Applications
ELISA FCM IHC-P
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human KLLN
Immunogen Range 20-120/178
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 100144748
Subcellular location Nucleus
Synonyms KILIN_HUMAN; Killin; killin, p53-regulated DNA replication inhibitor; KLLN.
Background DNA-binding protein involved in S phase checkpoint control-coupled apoptosis by mediating p53/TP53-induced apoptosis. Has the ability to inhibit DNA synthesis and S phase arrest coupled to apoptosis. Has affinity to both double- and single-stranded DNA.
Application Dilution
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Frequently asked questions

Which applications is KLLN Polyclonal Antibody (bs-6893R) validated for, and at what dilution?

bs-6893R is validated for ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does KLLN Polyclonal Antibody (bs-6893R) react with?

Tested reactivity: Human.

How should bs-6893R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise KLLN Polyclonal Antibody (bs-6893R)?

KLH conjugated synthetic peptide derived from human KLLN Immunogen range: 20-120/178.

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