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CENPT Polyclonal Antibody, Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
Overview
Catalog # bs-6901R-Cy7-100uL
Product Name CENPT Polyclonal Antibody, Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CENPT
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 80152
Swiss Prot Q96BT3
Subcellular location Nucleus
Synonyms C16orf56; CENP T; CENP-T; Cenpt; CENPT_HUMAN; Centromere protein T; Chromosome 16 open reading frame 56; ICEN22; Interphase centromere complex protein 22.
Background Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CENPT Polyclonal Antibody, Cy7 Conjugated (bs-6901R-Cy7) validated for, and at what dilution?

bs-6901R-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CENPT Polyclonal Antibody, Cy7 Conjugated (bs-6901R-Cy7) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bs-6901R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CENPT Polyclonal Antibody, Cy7 Conjugated (bs-6901R-Cy7)?

KLH conjugated synthetic peptide derived from human CENPT

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-6901R-Cy7)?

Cy7 on bs-6901R-Cy7: excitation / emission 743nm/767nm.