Septin 8 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6907R-Cy5 |
| Product Name | Septin 8 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Cow |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SEPT8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23176 |
| Subcellular location | Cytoplasm |
| Synonyms | Nkrf; RCG53214, isoform CRA_d; SEP2; SEP 2; SEP2; SEPT 8; SEPT8; SEPT-8; Septin8; Septin 8; Septin-8; SEPT8_HUMAN. |
| Background | This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. [provided by RefSeq, Jun 2012]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Septin 8 Polyclonal Antibody, Cy5 Conjugated (bs-6907R-Cy5) validated for, and at what dilution?
bs-6907R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Septin 8 Polyclonal Antibody, Cy5 Conjugated (bs-6907R-Cy5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow.
How should bs-6907R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Septin 8 Polyclonal Antibody, Cy5 Conjugated (bs-6907R-Cy5)?
KLH conjugated synthetic peptide derived from human SEPT8
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-6907R-Cy5)?
Cy5 on bs-6907R-Cy5: excitation / emission 625,650nm/670nm.