MICB Polyclonal Antibody

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-MICB Polyclonal Antibody, Unconjugated (bs-6933R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: Human HeLa cell lysates; Lane 2: Human U266 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human HUVEC cell lysates probed with MICB Polyclonal Antibody, Unconjugated (bs-6933R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HeLa cell lysates; Lane 2: Human U266 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human HUVEC cell lysates probed with MICB Polyclonal Antibody, Unconjugated (bs-6933R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Overview
Catalog #	bs-6933R
Product Name	MICB Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MICB
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	100507436, 4277
Swiss Prot	Q29983, Q29980
Subcellular location	Cell membrane
Synonyms	MICA: MHC class I polypeptide-related sequence A, MIC-A, HLA class I antigen MICB: MHC class I polypeptide-related sequence B, MIC-B, MHC class I mic-B antigen
Background	Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200