EBV Nuclear Antigen Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-6938R-Cy3 |
| Product Name | EBV Nuclear Antigen Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Virus |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from EBV Nuclear Antigen |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | EBNA1; EBNA-1; EBV nuclear antigen 1; Epstein Barr nuclear antigen 1; Epstein Barr virus; BKRF1; Epstein-Barr nuclear antigen 1; EBV nuclear antigen 1; |
| Background | Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is the one EBV antigen that is expressed in all EBV associated malignancies. It has long been thought to go undetected by the cell mediated immune system. However, recent studies show that EBNA1 can be presented to both CD4+ and CD8+ T cells, making it a potential new target for immunotherapy of EBV related cancers. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EBV Nuclear Antigen Polyclonal Antibody, Cy3 Conjugated (bs-6938R-Cy3) validated for, and at what dilution?
bs-6938R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EBV Nuclear Antigen Polyclonal Antibody, Cy3 Conjugated (bs-6938R-Cy3) react with?
Tested reactivity: Virus.
How should bs-6938R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EBV Nuclear Antigen Polyclonal Antibody, Cy3 Conjugated (bs-6938R-Cy3)?
KLH conjugated synthetic peptide derived from EBV Nuclear Antigen
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-6938R-Cy3)?
Cy3 on bs-6938R-Cy3: excitation / emission 512,550nm/570,615nm.