DHX32 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6944R-Cy5.5-100uL |
| Product Name | DHX32 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DHX32 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Ddx32; DEAD/H Asp-Glu-Ala-Asp/His box polypeptide 32; DEAD/H box 32; DEAD/H helicase like protein 1; DEAH Asp-Glu-Ala-His box polypeptide 32; DEAH box protein 32; DHLP1; DHX32; Helicase DDX32; HuDDX32; Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32; DHX32_HUMAN. |
| Background | DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX32 is a 743 amino acid nuclear protein that localizes to the mitochondria and is a member of the DEAD box helicase family. Expressed in various tissues, DDX32 is up-regulated by ionomycin in T lymphocytes and down-regulated in acute lymphoblastic leukemia. Considered a novel RNA helicase, DDX32 may play an important role in the development of colorectal cancer and may be involved in regulating T-cell response to certain apoptotic stimuli. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DHX32 Polyclonal Antibody, Cy5.5 Conjugated (BS-6944R-CY5-5) validated for, and at what dilution?
BS-6944R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DHX32 Polyclonal Antibody, Cy5.5 Conjugated (BS-6944R-CY5-5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Rabbit.
How should BS-6944R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DHX32 Polyclonal Antibody, Cy5.5 Conjugated (BS-6944R-CY5-5)?
KLH conjugated synthetic peptide derived from human DHX32
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6944R-CY5-5)?
Cy5.5 on BS-6944R-CY5-5: excitation / emission 675nm/694nm.