LCMV RING finger protein Z Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-6946R-Cy5.5-100uL |
| Product Name | LCMV RING finger protein Z Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Virus |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from LCMV Protein Z |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P18541 |
| Synonyms | Lymphocytic choriomeningitis virus.RING finger protein Z; Protein Z; Zinc-binding protein; Z_LYCVA. |
| Background | Plays a crucial role in virion assembly and budding. Expressed late in the virus life cycle, it acts as an inhibitor of viral transcription and RNA synthesis by interacting with the viral polymerase L (By similarity). Presumably recruits the NP encapsidated genome to cellular membranes at budding sites via direct interaction with NP. Plays critical roles in the final steps of viral release by interacting with host TSG101, a member of the vacuolar protein-sorting pathway and using other cellular host proteins involved in vesicle formation pathway. The budding of the virus progeny occurs after association of protein Z with the viral glycoprotein complex SSP-GP1-GP2 at the cell periphery, step that requires myristoylation of protein Z. Also selectively represses protein production by associating with host eIF4E |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is LCMV RING finger protein Z Polyclonal Antibody, Cy5.5 Conjugated (BS-6946R-CY5-5) validated for, and at what dilution?
BS-6946R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LCMV RING finger protein Z Polyclonal Antibody, Cy5.5 Conjugated (BS-6946R-CY5-5) react with?
Tested reactivity: Virus.
How should BS-6946R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LCMV RING finger protein Z Polyclonal Antibody, Cy5.5 Conjugated (BS-6946R-CY5-5)?
KLH conjugated synthetic peptide derived from LCMV Protein Z
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-6946R-CY5-5)?
Cy5.5 on BS-6946R-CY5-5: excitation / emission 675nm/694nm.