Factor B Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-6949R-PerCP-100uL |
| Product Name | Factor B Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Complement factor-B Bb fragment |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 629 |
| Swiss Prot | P00751 |
| Subcellular location | Secreted |
| Synonyms | BF; FB; BFD; GBG; CFAB; CFBD; PBF2; AHUS4; FBI12; H2-Bf; ARMD14; Complement factor B; C3/C5 convertase; Glycine-rich beta glycoprotein; Properdin factor B; CFB |
| Background | Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Factor B Polyclonal Antibody, PerCP Conjugated (bs-6949R-PerCP) validated for, and at what dilution?
bs-6949R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Factor B Polyclonal Antibody, PerCP Conjugated (bs-6949R-PerCP) react with?
Tested reactivity: Mouse, Rat.
How should bs-6949R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Factor B Polyclonal Antibody, PerCP Conjugated (bs-6949R-PerCP)?
KLH conjugated synthetic peptide derived from human Complement factor-B Bb fragment
What are the excitation and emission wavelengths of the PerCP conjugate (bs-6949R-PerCP)?
PerCP on bs-6949R-PerCP: excitation / emission 482nm/677nm.