Ep300 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6954r-bf350 |
| Product Name | Ep300 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EP300 inhibitory protein 1 |
| Immunogen Range | 1451-1600/2414 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CREBBP/EP300 inhibitory protein 1; Cyclic AMP responsive enhancer binding protein; E1A associated protein p300; E1A binding protein p300; E1A-associated protein p300; EC 2.3.1.48; EP300; EP300: E1A binding protein p300; EP300_HUMAN; istone acetyltransferase p300; 300 HAT; RB and P300 binding protein EID 1; Retinoblastoma protein associated protein; RSTS2. |
| Background | Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Ep300 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-6954R-BF350) validated for, and at what dilution?
bs-6954R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Ep300 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-6954R-BF350) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse.
How should bs-6954R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Ep300 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-6954R-BF350)?
KLH conjugated synthetic peptide derived from human EP300 inhibitory protein 1 Immunogen range: 1451-1600/2414.
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-6954R-BF350)?
AbBy Fluor-350 on bs-6954R-BF350: excitation / emission 346nm/442nm.