Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

phospho-Estrogen Receptor beta (Ser87) Polyclonal Antibody

Mouse testis lysates probed with phospho-Estrogen Receptor beta (Ser87) Polyclonal Antibody, Unconjugated (bs-6959R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Mouse testis lysates probed with phospho-Estrogen Receptor beta (Ser87) Polyclonal Antibody, Unconjugated (bs-6959R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Testis cell lysates; Lane 2: Mouse Ovary cell lysates; Lane 3: Mouse Uterus cell lysates probed with Estrogen Receptor beta (Ser87) Polyclonal Antibody, Unconjugated (bs-6959R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Mouse Testis cell lysates; Lane 2: Mouse Ovary cell lysates; Lane 3: Mouse Uterus cell lysates probed with Estrogen Receptor beta (Ser87) Polyclonal Antibody, Unconjugated (bs-6959R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-Estrogen Receptor beta (Ser87)) polyclonal Antibody, unconjugated (bs-6959R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-Estrogen Receptor beta (Ser87)) polyclonal Antibody, unconjugated (bs-6959R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-Estrogen Receptor beta (Ser87) antibody (bs-6959R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-Estrogen Receptor beta (Ser87) antibody (bs-6959R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Sheep
  • Rabbit
Overview
Catalog # bs-6959R
Product Name phospho-Estrogen Receptor beta (Ser87) Polyclonal Antibody
Applications
WB ELISA
Reactivity Mouse
Predicted Reactivity Human, Rat, Sheep, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human Estrogen Receptor beta around the phosphorylation site of Ser87 [HL(p-S)PL]
Modification Site Ser87
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2100
Subcellular location Nucleus
Synonyms Estrogen Receptor beta phospho S87; Estrogen Receptor beta phospho Ser87; p-ER betaS87; p-ER betaSer87; ER-betaSer87; ER-betaS87; Estrogen receptor-beta; ER BETA; ER-BETA; ER-beta; Erb; ESR 2; ESR 2; ESR B; ESR-B; ESR BETA; ESR-BETA; ESR2; ESR2; ESR2_HUMAN; ESRB; ESTRB; estrogen nuclear receptor beta variant a; estrogen nuclear receptor beta variant b; estrogen receptor 2 ER beta; Estrogen Receptor 2; Estrogen Receptor-2; estrogen receptor beta 4; Estrogen receptor beta; NR3A2; Nuclear receptor subfamily 3 group A member 2.
Background Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER alpha and ER beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ER alpha and ER beta A have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER alpha and ER beta may be regulated by distinct mechanisms even though they share many functional characteristics.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000

Frequently asked questions

Which applications is phospho-Estrogen Receptor beta (Ser87) Polyclonal Antibody (bs-6959R) validated for, and at what dilution?

bs-6959R is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-Estrogen Receptor beta (Ser87) Polyclonal Antibody (bs-6959R) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Sheep, Rabbit.

How should bs-6959R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-Estrogen Receptor beta (Ser87) Polyclonal Antibody (bs-6959R)?

KLH conjugated synthetic phosphopeptide derived from human Estrogen Receptor beta around the phosphorylation site of Ser87 [HL(p-S)PL]

Search our catalog of hundreds of thousands of products