RNA polymerase II Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6972R-BF555-100uL |
| Product Name | RNA polymerase II Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5430 |
| Swiss Prot | P24928 |
| Subcellular location | Nucleus |
| Synonyms | POLR2A; POLR2; DNA directed RNA polymerase II A; DNA-directed RNA polymerase II largest subunit; DNA-directed RNA polymerase II subunit A; RPB1_HUMAN. |
| Background | RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RNA polymerase II Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6972R-BF555) validated for, and at what dilution?
bs-6972R-BF555 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RNA polymerase II Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6972R-BF555) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse.
How should bs-6972R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RNA polymerase II Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-6972R-BF555)?
KLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-6972R-BF555)?
AbBy Fluor-555 on bs-6972R-BF555: excitation / emission 553nm/568nm.