Phospholipase C beta 1 Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-6976R-Cy5-100uL |
| Product Name | Phospholipase C beta 1 Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Phospholipase C beta 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23236 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | 1-phosphatidylinositol-45-bisphosphate phosphodiesterase beta 1; 1vphosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1 ; Phosphb; Phosphoinositide phospholipase C; Phosphoinositide phospholipase C-beta 1; Phospholipase C beta 1 phosphoinositide-specic; Phospholipase C I; PI PLC; PLC 1; PLC_1; PLC-154; PLC-beta-1; PLC-I; PLC154; Plcb; PLCB1; Plcb1 protein; PLCbeta1. |
| Background | Phospholipase C beta 1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. Its gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for its gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospholipase C beta 1 Antibody, Cy5 Conjugated (bs-6976R-Cy5) validated for, and at what dilution?
bs-6976R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospholipase C beta 1 Antibody, Cy5 Conjugated (bs-6976R-Cy5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Horse, Chicken.
How should bs-6976R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospholipase C beta 1 Antibody, Cy5 Conjugated (bs-6976R-Cy5)?
KLH conjugated synthetic peptide derived from human Phospholipase C beta 1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-6976R-Cy5)?
Cy5 on bs-6976R-Cy5: excitation / emission 625,650nm/670nm.