AIF1/Iba1 Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70004r |
| Product Name | AIF1/Iba1 Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~17 kDa AIF1/Iba1 protein. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Full-length human AIF1/Iba1 protein expressed in and purified from E. coli. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 199 |
| Swiss Prot | P55008 |
| Background | AIF1, allograft inflammatory factor-1, (also known as Iba1, Ionized calcium binding adaptor molecule 1) is a 17 kDa protein that is a pro-inflammatory molecule that mediates calcium signals (Ito, D. et al 1998). During inflammation, increased expression of AIF1 occurs in microglia, macrophages, T-cells, synoviocytes, and adipocytes (Watano, K., et al, 2001). Expression of AIF-1 has been observed in healthy brains and varies throughout the day, gaining the highest expression in the prefrontal and parietal cortices when sleeping (Romcy-Pereira, R.N, et al, 2009). Increased expression of AIF1 has been observed in several diseases including breast cancer (Liu, S., et al 2008), atherosclerosis (Utans, U., et al, 1995), and rheumatoid arthritis (Kimura, M., et al, 2007). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is AIF1/Iba1 Antibody (bs-70004R) validated for, and at what dilution?
bs-70004R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AIF1/Iba1 Antibody (bs-70004R) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-70004R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise AIF1/Iba1 Antibody (bs-70004R)?
Full-length human AIF1/Iba1 protein expressed in and purified from E. coli.