Amyloid Precursor Protein Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70015r |
| Product Name | Amyloid Precursor Protein Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~115 kDa APP protein. Immunolabeling of the APP protein band is completely blocked by preadsorption of the antibody with the peptide used as antigen. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Chicken, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat APP, conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 54226 |
| Swiss Prot | P08592 |
| Synonyms | A4 amyloid protein antibody, A4_HUMAN antibody, AAA antibody, ABETA antibody, ABPP antibody, AD1 antibody, AICD-50 antibody, AICD-57 antibody, AICD-59 antibody, AID(50) antibody, AID(57) antibody, AID(59) antibody, Alzheimer disease amyloid protein antibody, Amyloid beta (A4) precursor protein antibody, Amyloid beta A4 protein antibody, Amyloid beta protein antibody, Amyloid intracellular domain 50 antibody, Amyloid intracellular domain 57 antibody, Amyloid intracellular domain 59 antibody, Amyloid precursor protein antibody, APP antibody, APPI antibody, Beta amyloid peptide antibody, Beta-amyloid precursor protein antibody, Beta-APP40 antibody, Beta-APP42 antibody, C31 antibody, Cerebral vascular amyloid peptide antibody, CTFgamma antibody, CVAP antibody, Gamma-CTF(50) antibody, Gamma-CTF(57) antibody, Gamma-CTF(59) antibody, peptidase nexin II antibody, PN 2 antibody, PN II antibody, PN-II antibody, PN2 antibody, PreA4 antibody, Protease nexin II antibody, Protease nexin-II antibody, S-APP-alpha antibody, S-APP-beta antibody |
| Background | A large body of evidence has implicated the amyloid precursor protein (APP) in the pathogenesis of Alzheimer's disease (AD) (Kamenetz et al., 2003). The phosphorylation of APP at Thr668 is thought to play a critical role in generation of the soluble APP (beta) and beta-amyloid peptide (abeta) which are the major components of senile plaques in patient brains inflicted with AD (Liu et al., 2003; Ando et al., 2001). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Amyloid Precursor Protein Antibody (bs-70015R) validated for, and at what dilution?
bs-70015R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Amyloid Precursor Protein Antibody (bs-70015R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Chicken, Non-Human Primate.
How should bs-70015R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Amyloid Precursor Protein Antibody (bs-70015R)?
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat APP, conjugated to keyhole limpet hemocyanin (KLH).