Aquaporin 2 (Ser269) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70019r |
| Product Name | Aquaporin 2 (Ser269) Antibody |
| Applications |
WB
IP
IHC
|
| Specificity | Specific for endogenous levels of the ~29 kDa AQP2 protein phosphorylated at Ser269. Also recognizes the glycosylated form of AQP2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Bovine |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269 of rat aquaporin 2, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser269 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 25386 |
| Swiss Prot | P34080 |
| Synonyms | ADH water channel antibody, AQP 2 antibody, AQP CD antibody, AQP-2 antibody, AQP-CD antibody, AQP2 antibody, AQP2_HUMAN antibody, AQPCD antibody, Aquaporin 2 collecting duct antibody, Aquaporin CD antibody, Aquaporin-2 antibody, Aquaporin-CD antibody, Aquaporin2 antibody, Aquaporine 2 antibody, Collecting duct water channel protein antibody, MGC34501 antibody, Water channel aquaporin 2 antibody, Water channel protein for renal collecting duct antibody, WCH CD antibody, WCH-CD antibody, WCHCD antibody |
| Background | Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al., 2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Serine-269 has been recently identified as a vasopressin-mediated phosphorylation site on AQP2 and as such has shown to potentiate plasma membrane retention of AQP2 (Hoffert JD et al., 2008). |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
| IHC | |