Calretinin Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70029s |
| Product Name | Calretinin Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~29 kDa calretinin protein in Western blots of rat brain lysate. The antibody also works well for immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections. |
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Sheep |
| Source | Purified guinea pig calretinin. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 10 mM Tris, 100 mM NaCl |
| Storage Condition | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy |
| Target | |
| Gene ID | 117059 |
| Swiss Prot | P47728 |
| Synonyms | 29 kDa calbindin antibody, CAB 29 antibody, CAB29 antibody, CAL 2 antibody, CAL2 antibody, CALB 2 antibody, CALB2 antibody, CALB2_HUMAN antibody, Calbindin 2 29kDa antibody, Calbindin 2 antibody, Calbindin D29K antibody, Calbindin2 antibody, Calretinin antibody, CR antibody |
| Background | Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |
Frequently asked questions
Which applications is Calretinin Antibody (bs-70029S) validated for, and at what dilution?
bs-70029S is validated for WB (1:300-5000); IHC. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Calretinin Antibody (bs-70029S) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Non-Human Primate.
How should bs-70029S be stored?
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy Storage buffer: 10 mM Tris, 100 mM NaCl
What immunogen was used to raise Calretinin Antibody (bs-70029S)?
Purified guinea pig calretinin.