CK1Mt (Tyr153) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70042r-100uL |
| Product Name | CK1Mt (Tyr153) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~46 kDa CK1Mt protein phosphorylated at Tyr153. Immunolabeling of CK1Mt is greatly decreased by lambda-phosphatase treatment. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Bovine, Sheep, Rabbit, Guinea Pig, Hamster, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr153 of human CK1Mt, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Tyr153 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 1159 |
| Swiss Prot | P12532 |
| Synonyms | Acidic type mitochondrial creatine kinase antibody, CKMT antibody, CKMT1 antibody, CKMT1A antibody, CKMT1B antibody, Creatine kinase mitochondrial 1 ubiquitous antibody, Creatine kinase U type mitochondrial antibody, Creatine kinase ubiquitous mitochondrial antibody, Creatine kinase mitochondrial 1 (ubiquitous) antibody, Creatine kinase mitochondrial 1A antibody, Creatine kinase mitochondrial 1B antibody, Mia CK antibody, U MtCK antibody, Ubiquitous mitochondrial creatine kinase antibody, UMTCK antibody |
| Background | Creatine kinase (CK1) plays an important role transferring phosphate groups from phosphocreatine to ADP in the cytosol of tissues having high, fluctuating energy demands like skeletal muscle, heart, and brain. Mitochondrial CK1, CK1Mt, has two isoforms, sarcomeric CK1Mt and ubiquitous CK1Mt. CK1Mt is localized between the MIM (mitochondrial inner membrane) and MOM (mitochondrial outer membrane) and bound to the cardiolipin-rich inner leaflet (Muller et al., 1985) and along the cristae membranes (Wegmann et al., 1991). CK1Mt exists in two forms; a homo dimer and an octamer consisting of four homodimers. The CK1Mt octamer forms permanent contact sites and maintains a complex structure including porin and ANT (adenine nucleotide translocase) within MIM and MOM (Speer et al, 2005). There have been several phospho-serine, threonine, and tyrosine sites identified within CK1Mt, the role of each one has yet to be determined. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CK1Mt (Tyr153) Antibody (bs-70042R) validated for, and at what dilution?
bs-70042R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CK1Mt (Tyr153) Antibody (bs-70042R) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Bovine, Sheep, Rabbit, Guinea Pig, Hamster, Non-Human Primate.
How should bs-70042R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise CK1Mt (Tyr153) Antibody (bs-70042R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr153 of human CK1Mt, conjugated to keyhole limpet hemocyanin (KLH).