PRAME Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-7004R-Cy5.5-100uL |
| Product Name | PRAME Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PRAME |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23532 |
| Swiss Prot | P78395 |
| Subcellular location | Nucleus, Cell membrane |
| Synonyms | MAPE; OIP4; CT130; OIP-4; Melanoma antigen preferentially expressed in tumors; Opa-interacting protein 4; Preferentially expressed antigen of melanoma; PRAME |
| Background | Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PRAME Polyclonal Antibody, Cy5.5 Conjugated (BS-7004R-CY5-5) validated for, and at what dilution?
BS-7004R-CY5-5 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PRAME Polyclonal Antibody, Cy5.5 Conjugated (BS-7004R-CY5-5) react with?
Tested reactivity: Human, Mouse.
How should BS-7004R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PRAME Polyclonal Antibody, Cy5.5 Conjugated (BS-7004R-CY5-5)?
KLH conjugated synthetic peptide derived from human PRAME
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-7004R-CY5-5)?
Cy5.5 on BS-7004R-CY5-5: excitation / emission 675nm/694nm.