Connexin 43 (Ser368) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70051r-100uL |
| Product Name | Connexin 43 (Ser368) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~43 kDa connexin-43 protein phosphorylated at Ser368. Immunolabeling is completely eliminated by treatment with λ-phosphatase. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Bovine, Chicken, Guinea Pig, Zebrafish |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser368 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 24392 |
| Swiss Prot | P08050 |
| Synonyms | Connexin 43 antibody, Connexin-43 antibody, Cx 43 antibody, Cx43 antibody, CXA1_HUMAN antibody, DFNB38 antibody, Gap junction 43 kDa heart protein antibody, Gap junction alpha-1 protein antibody, Gap junction protein alpha 1 43kDa (connexin 43) antibody, Gap junction protein alpha 1 43kDa antibody, Gap junction protein alpha like antibody, GJA 1 antibody, Gja1 antibody, GJAL antibody, ODD antibody, ODDD antibody, ODOD antibody, SDTY3 antibody |
| Background | Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser-368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser-368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Connexin 43 (Ser368) Antibody (bs-70051R) validated for, and at what dilution?
bs-70051R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Connexin 43 (Ser368) Antibody (bs-70051R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Bovine, Chicken, Guinea Pig, Zebrafish.
How should bs-70051R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Connexin 43 (Ser368) Antibody (bs-70051R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to keyhole limpet hemocyanin (KLH).