COXIV (Ser58) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70052r |
| Product Name | COXIV (Ser58) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~17 kDa COXIV-1 protein phosphorylated at Ser58. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. |
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of mouse COXIV, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser58 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 12857 |
| Swiss Prot | P19783 |
| Synonyms | AL024441 antibody, COX 4 antibody, COX IV 1 antibody, COX IV antibody, COX IV-1 antibody, Cox4 antibody, COX41_HUMAN antibody, Cox4a antibody, COX4B antibody, COX4I1 antibody, COX4I2 antibody, COX4L2 antibody, Cytochrome c oxidase polypeptide IV antibody, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody, Cytochrome C Oxidase subunit IV antibody, Cytochrome c oxidase subunit IV isoform 1 antibody, Cytochrome c oxidase subunit IV isoform 2 (lung) antibody, Cytochrome c oxydase subunit 4 antibody, MGC105470 antibody, MGC72016 antibody |
| Background | COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms; COXIV-1 and COXIV-2 (Huttermann et al., 2001). COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart (Huttermann et al., 2001). Phosphorylation of amino acid residue Ser-58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation (AcinPerez et al., 2011). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is COXIV (Ser58) Antibody (bs-70052R) validated for, and at what dilution?
bs-70052R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does COXIV (Ser58) Antibody (bs-70052R) react with?
Tested reactivity: Mouse, Rat.
How should bs-70052R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise COXIV (Ser58) Antibody (bs-70052R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of mouse COXIV, conjugated to keyhole limpet hemocyanin (KLH).