DENND3 (Thr450) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70062r |
| Product Name | DENND3 (Thr450) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~142 kDa DENND3 phosphorylated at Thr450. The immunolabeling is completely eliminated by treatment with λ-phosphatase. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr450 of human DENND3, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Thr450 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 315055 |
| Swiss Prot | A2RUS2 |
| Synonyms | DENN Domain Containing 3, DENN Domain-Containing Protein 3, DENN/MADD Domain Containing 3, KIAA0870 DENN/MADD domain containing 3, KIAA0870DENN domain-containing protein 3 |
| Background | The DENN (differentially expressed in normal and neoplastic cells) domain (DENND) is a poorly characterized protein module conserved throughout evolution (McPherson et al., 2011). Proteins bearing a DENN domain have recently emerged as the largest family of Rab GEFs. Among these DENN domain proteins, DENND3 is a GEF for Rab12; promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB12 into its active GTP-bound form (McPherson and Xu, 2015; Matsui et al., 2011). |
| Application Dilution | |
| WB | 1:300-5000 |