Dynamin Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70074r |
| Product Name | Dynamin Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~95 kDa dynamin protein. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Chicken, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide corresponding to amino acid residues from the N-terminal region of human dynamin, conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 1759 |
| Swiss Prot | Q05193 |
| Synonyms | B dynamin antibody, D100 antibody, DNM 1 antibody, DNM antibody, DNM1 antibody, DYN1_HUMAN antibody, Dynamin antibody, Dynamin-1 antibody, Dynamin1 antibody |
| Background | Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). There are 3 known isoforms of Dynamin, each having several splice variants as well. Dynamin I is expressed only in neurons whereas Dynamin II is ubiquitously expressed and Dynamin III is found primarily in the testes. Dynamin 1 is phosphorylated by PKC and dephosphorylated by calcineurin. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Dynamin Antibody (bs-70074R) validated for, and at what dilution?
bs-70074R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Dynamin Antibody (bs-70074R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Bovine, Chicken, Non-Human Primate.
How should bs-70074R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Dynamin Antibody (bs-70074R)?
Synthetic peptide corresponding to amino acid residues from the N-terminal region of human dynamin, conjugated to keyhole limpet hemocyanin (KLH).