Dynamin (Ser778) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70076s |
| Product Name | Dynamin (Ser778) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~95 kDa dynamin protein phosphorylated at Ser778. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is completely eliminated by treatment with λ-phosphatase. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Bovine, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Sheep |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 of rat dynamin, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser778 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 140694 |
| Swiss Prot | P21575 |
| Synonyms | B dynamin antibody, D100 antibody, DNM 1 antibody, DNM antibody, DNM1 antibody, DYN1_HUMAN antibody, Dynamin antibody, Dynamin-1 antibody, Dynamin1 antibody |
| Background | Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser-774 and Ser-778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Dynamin (Ser778) Antibody (bs-70076S) validated for, and at what dilution?
bs-70076S is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Dynamin (Ser778) Antibody (bs-70076S) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Bovine, Chicken.
How should bs-70076S be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Dynamin (Ser778) Antibody (bs-70076S)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 of rat dynamin, conjugated to keyhole limpet hemocyanin (KLH).