eEF1A2 (Ser358) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70077r-100uL |
| Product Name | eEF1A2 (Ser358) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~50 kDa eEF1A2 phosphorylated at Ser358. Immunolabeling is completely eliminated by treatment with λ-phosphatase. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Sheep, Chicken, Xenopus, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser358 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 1917 |
| Swiss Prot | Q05639 |
| Synonyms | eEF1A-2 antibody, Eef1a2 antibody, EEF1AL antibody, EF 1 alpha 2 antibody, EF-1-alpha-2 antibody, EF1A antibody, EF1A2_HUMAN antibody, Elongation factor 1 A 2 antibody, Elongation factor 1 alpha antibody, Elongation factor 1 alpha 2 antibody, Elongation factor 1-alpha 2 antibody, Eukaryotic elongation factor 1 A-2 antibody, Eukaryotic translation elongation factor 1 alpha 2 antibody, FLJ41696 antibody, HS1 antibody, OTTHUMP00000031776 antibody, Statin like antibody, Statin S1 antibody, Statin-S1 antibody, STN antibody, STNL antibody |
| Background | Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993). Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002). Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009). Ribosome-associated JNK phosphorylates Ser-358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is eEF1A2 (Ser358) Antibody (bs-70077R) validated for, and at what dilution?
bs-70077R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eEF1A2 (Ser358) Antibody (bs-70077R) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Sheep, Chicken, Xenopus, Non-Human Primate.
How should bs-70077R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise eEF1A2 (Ser358) Antibody (bs-70077R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2, conjugated to keyhole limpet hemocyanin (KLH).