eIF4A2 Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70078r |
| Product Name | eIF4A2 Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~46 kDa eIF4A2 protein. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Bovine, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide from the N-terminal region of the human eIF4A2 protein. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 1917 |
| Swiss Prot | Q14240 |
| Synonyms | ATP dependent RNA helicase eIF4A 2 antibody, ATP-dependent RNA helicase eIF4A-2 antibody, BM-010 antibody, DDX2B antibody, eIF 4A II antibody, eIF-4A-II antibody, EIF4A antibody, eIF4A II antibody, eIF4A-II antibody, EIF4A2 antibody, EIF4F antibody, Eukaryotic initiation factor 4A II antibody, Eukaryotic initiation factor 4A-II antibody, eukaryotic translation initiation factor 4A isoform 2 antibody, Eukaryotic translation initiation factor 4A2 antibody, IF4A2_HUMAN antibody, N-terminally processed antibody |
| Background | The eIF4A, eukaryotic initiation factor 4A, are a set of proteins, eIF4A1, eIF4A2, and eIF4A3, that belong to the extensive DEAD-box RNA helicase family (Weinstein et al., 1997). They are involved in many aspects of RNA metabolism by virtue of their RNA-binding capacity and ATPase activity (Lu et al., 2014). eIF4A2 is a highly conserved gene for one of the protein-synthesis initiation factors involved in the binding of mRNA to the ribosome (Gingras et al., 1999). In humans eIF4A2 is expressed in all normal tissues, though highly expressed in skeletal muscle (Sudo et al., 1995). Studies suggest eIF4A2 may play a role in some cancers, specifically breast and lung cancer, along with melanoma (Shaoyan et al., 2013). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is eIF4A2 Antibody (bs-70078R) validated for, and at what dilution?
bs-70078R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does eIF4A2 Antibody (bs-70078R) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Bovine, Non-Human Primate.
How should bs-70078R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise eIF4A2 Antibody (bs-70078R)?
Synthetic peptide from the N-terminal region of the human eIF4A2 protein.