ERK/MAPK (Thr202/Tyr204) Antibody

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Bovine
Chicken
Zebrafish
Xenopus
Non-Human Primate

Overview
Catalog #	bs-70084r
Product Name	ERK/MAPK (Thr202/Tyr204) Antibody
Applications	WB, IHC
Specificity	Specific for endogenous levels of the ~42 - 44 kDa ERK/MAPK protein phosphorylated at Thr202 and Tyr204. Immunolabeling is completely eliminated by treatment with _-phosphatase.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Bovine, Chicken, Zebrafish, Xenopus, Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 of rat ERK/MAPK conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Thr202, Tyr204
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 g per ml BSA and 50% glycerol.
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Gene ID	116590
Swiss Prot	P63086
Synonyms	ERK 1 antibody, ERK 2 antibody, ERK-2 antibody, ERK1 antibody, erk1/2 antibody, ERK2 antibody, ERT1 antibody, ERT2 antibody, Extracellular signal regulated kinase 1 antibody, Extracellular signal-regulated kinase 2 antibody, MAP kinase 1 antibody, MAP kinase 2 antibody, MAP kinase isoform p42 antibody, MAP kinase isoform p44 antibody, MAPK 1 antibody, MAPK 2 antibody, MAPK 3 antibody, Mapk1 antibody, MAPK2 antibody, MAPK3 antibody, Mitogen-activated protein kinase 1 antibody, Mitogen-activated protein kinase 2 antibody, MK01_HUMAN antibody, p38 antibody, p40 antibody, p41 antibody, p42-MAPK antibody, PRKM 2 antibody
Background	Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).
Application Dilution
WB	1:300-5000
IHC