FANCI (Ser556) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70087r-100uL |
| Product Name | FANCI (Ser556) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~150 kDa FANCI protein phosphorylated at Ser556. Immunolabeling is completely eliminated by treatment with λ-phosphatase. |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Sheep, Goat, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser556 of human FANCI, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser556 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 55215 |
| Swiss Prot | Q9NVI1 |
| Synonyms | FANCI antibody, FANCI gene antibody, FANCI_HUMAN antibody, Fanconi anemia group I protein antibody, Fanconi anemia complementation group I antibody, FLJ10719 antibody, FLJ14658 antibody, KIAA1794 antibody, Protein FACI antibody, Protein FANCI antibody, |
| Background | Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al, 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitination by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation at Ser-556 has been shown to play a key role in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is FANCI (Ser556) Antibody (bs-70087R) validated for, and at what dilution?
bs-70087R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FANCI (Ser556) Antibody (bs-70087R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Bovine, Sheep, Goat, Non-Human Primate.
How should bs-70087R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise FANCI (Ser556) Antibody (bs-70087R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser556 of human FANCI, conjugated to keyhole limpet hemocyanin (KLH).