GABAA Receptor _3 Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70098r |
| Product Name | GABAA Receptor _3 Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~51 kDa α3-subunit of the GABAA receptor. Immunolabeling is absent in α3-subunit knockout animals. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Bovine, Zebrafish, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide from the N-terminal region of the α3 subunit of rat GABAA receptor. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 24947 |
| Swiss Prot | P20236 |
| Synonyms | GABA A Receptor alphα3 antibody, GABA(A) receptor subunit alphα3 antibody, GABA(A) receptor subunit alpha-3 antibody, GABR A3 antibody, GABRα3 antibody, Gabra3 antibody, Gamma aminobutyric acid (GABA) A receptor alphα3 antibody, Gamma aminobutyric acid A receptor alphα3 antibody, Gamma aminobutyric acid receptor subunit alphα3 antibody, Gamma-aminobutyric acid receptor subunit alpha-3 antibody, GBRA3_HUMAN antibody, MGC33793 antibody |
| Background | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |
Frequently asked questions
Which applications is GABAA Receptor _3 Antibody (bs-70098R) validated for, and at what dilution?
bs-70098R is validated for WB (1:300-5000); IHC. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GABAA Receptor _3 Antibody (bs-70098R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Bovine, Zebrafish, Non-Human Primate.
How should bs-70098R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise GABAA Receptor _3 Antibody (bs-70098R)?
Synthetic peptide from the N-terminal region of the α3 subunit of rat GABAA receptor.