GABAA Receptor _, N-Terminus Antibody

Applications

Reactivity

Mouse
Rat

Overview
Catalog #	bs-70107r
Product Name	GABAA Receptor _, N-Terminus Antibody
Applications	WB, IHC
Specificity	Specific for endogenous levels of the ~50 kDa _-subunit of the GABAA receptor.
Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Fusion protein from the N-terminus of the _ subunit of rat GABAA receptor.
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 g per ml BSA and 50% glycerol.
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Gene ID	29689
Swiss Prot	P18506
Synonyms	GABA(A) receptor subunit delta antibody, Gabrd antibody, Gamma aminobutyric acid GABA A receptor delta antibody, Gamma aminobutyric acid receptor delta subunit precursor GABA A receptor antibody, Gamma-aminobutyric acid receptor subunit delta antibody, GBRD_HUMAN antibody, MGC45284 antibody
Background	Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six _s, four _s and four _s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin,1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for _- and _-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, co-expression of a _-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pltl et al., 2003). More recently there have been a number of studies demonstrating that the _-subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002).
Application Dilution
WB	1:300-5000
IHC