GABAA Receptor _2 Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70108r |
| Product Name | GABAA Receptor _2 Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~46 kDa γ2-subunit of the GABAA receptor. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Chicken, Zebrafish, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide corresponding to amino acid residues specific to the γ2 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Neat Pooled whole antiserum |
| Storage Buffer | Neat whole antiserum |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 29709 |
| Swiss Prot | P18508 |
| Synonyms | CAE 2 antibody, CAE2 antibody, ECA 2 antibody, ECA2 antibody, GABA(A) receptor gamma 2 antibody, GABA(A) receptor subunit gamma 2 antibody, GABA(A) receptor subunit gamma-2 antibody, GABRG 2 antibody, GABRγ2 antibody, Gamma aminobutyric acid (GABA) A receptor gamma 2 antibody, Gamma aminobutyric acid A receptor gamma 2 antibody, Gamma aminobutyric acid receptor gamma 2 subunit antibody, Gamma-aminobutyric acid receptor subunit gamma-2 antibody, GBRG2_HUMAN antibody, GEFSP 3 antibody, GEFSP3 antibody |
| Background | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, co-expression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of β-subunits of the receptor has been shown to modulate GABA-A-R function (Brandon et al., 2003). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |
Frequently asked questions
Which applications is GABAA Receptor _2 Antibody (bs-70108R) validated for, and at what dilution?
bs-70108R is validated for WB (1:300-5000); IHC. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GABAA Receptor _2 Antibody (bs-70108R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Chicken, Zebrafish, Non-Human Primate.
How should bs-70108R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: Neat whole antiserum
What immunogen was used to raise GABAA Receptor _2 Antibody (bs-70108R)?
Synthetic peptide corresponding to amino acid residues specific to the γ2 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).