GABAA Receptor _2 (Ser327) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70110r |
| Product Name | GABAA Receptor _2 (Ser327) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~45 kDa GABAA receptor γ2 subunit phosphorylated at Ser327. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Bovine, Chicken, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 of the γ2 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser327 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 29709 |
| Swiss Prot | P18508 |
| Synonyms | CAE 2 antibody, CAE2 antibody, ECA 2 antibody, ECA2 antibody, GABA(A) receptor gamma 2 antibody, GABA(A) receptor subunit gamma 2 antibody, GABA(A) receptor subunit gamma-2 antibody, GABRγ2 antibody, GABRG2 antibody, Gamma aminobutyric acid (GABA) A receptor gamma 2 antibody, Gamma aminobutyric acid A receptor gamma 2 antibody, Gamma aminobutyric acid receptor gamma 2 subunit antibody, Gamma-aminobutyric acid receptor subunit gamma-2 antibody, GBRG2_HUMAN antibody, GEFSP 3 antibody, GEFSP3 antibody |
| Background | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-AB subtype of receptors. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. It has recently been suggested that PKCε regulates the sensitivity of GABA-A α1β2γ2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of γ2 (Qi et al., 2007) |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is GABAA Receptor _2 (Ser327) Antibody (bs-70110R) validated for, and at what dilution?
bs-70110R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GABAA Receptor _2 (Ser327) Antibody (bs-70110R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Bovine, Chicken, Non-Human Primate.
How should bs-70110R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise GABAA Receptor _2 (Ser327) Antibody (bs-70110R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 of the γ2 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).