GABA Transporter (GAT) 1 Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70113r |
| Product Name | GABA Transporter (GAT) 1 Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~67 kDa GAT-1 protein. Immunolabeling is blocked by pre-adsorption of antibody with the peptide used to generate the antibody. |
| Reactivity | Human, Mouse, Rat, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat GAT1, conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Affinity purification |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 79212 |
| Swiss Prot | P23978 |
| Synonyms | GABATHG antibody, GABATR antibody, GABT 1 antibody, GABT1 antibody, GAT-1 antibody, GAT1 antibody, SC6A1_HUMAN antibody, Slc6a1 antibody, Sodium and chloride dependent GABA transporter 1 antibody, Sodium- and chloride-dependent GABA transporter 1 antibody, Solute carrier family 6 (neurotransmitter transporter GABA) member 1 antibody, Solute carrier family 6 member 1 antibody |
| Background | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |
Frequently asked questions
Which applications is GABA Transporter (GAT) 1 Antibody (bs-70113R) validated for, and at what dilution?
bs-70113R is validated for WB (1:300-5000); IHC. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GABA Transporter (GAT) 1 Antibody (bs-70113R) react with?
Tested reactivity: Human, Mouse, Rat, Non-Human Primate.
How should bs-70113R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise GABA Transporter (GAT) 1 Antibody (bs-70113R)?
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat GAT1, conjugated to keyhole limpet hemocyanin (KLH).