GluR2-subunit (Ser880) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70125r |
| Product Name | GluR2-subunit (Ser880) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~100 kDa GluR2 protein phosphorylated at Ser880. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. |
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Chicken, Zebrafish, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser880 conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser880 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 29627 |
| Swiss Prot | P19491 |
| Synonyms | AMPA 2 antibody, AMPA selective glutamate receptor 2 antibody, AMPA-selective glutamate receptor 2 antibody, AMPA2 antibody, GluA2 antibody, GLUR 2 antibody, GLUR B antibody, GluR K2 antibody, GluR-2 antibody, GluR-B antibody, GluR-K2 antibody, GLUR2 antibody, GLURB antibody, Glutamate receptor 2 antibody, Glutamate receptor ionotropic AMPA 2 antibody, Glutamate receptor ionotropic antibody, Gria2 antibody, GRIA2_HUMAN antibody, HBGR2 antibody |
| Background | The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The number of GluR2 subunits in the AMPA receptor complex affects the Ca2+ permeability, rectification and single-channel conductance of AMPA receptors. Ser-880 has been identified as the PKC phosphorylation site within the C-terminal region of GluR2 and has been shown to differentially regulate the interaction of the PDZ domain-containing proteins GRIP1 and PICK 1 (Matsuda et al., 1999) |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is GluR2-subunit (Ser880) Antibody (bs-70125R) validated for, and at what dilution?
bs-70125R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GluR2-subunit (Ser880) Antibody (bs-70125R) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Chicken, Zebrafish, Non-Human Primate.
How should bs-70125R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise GluR2-subunit (Ser880) Antibody (bs-70125R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser880 conjugated to keyhole limpet hemocyanin (KLH).