Glycine Receptor Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70127r-200ug |
| Product Name | Glycine Receptor Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~48 kDa α1- and α2-subunits of the glycine receptor. Immunolabeling is blocked by preadsorption of the antibody with the peptide immunogen. Does not recognize other glycine receptor subunits. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide corresponding to amino acid residues from the N-terminal region of the α1 subunit of rat glycine, conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | Lyophilized from 5 mM ammonium biocarbonate. |
| Storage Condition | The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working |
| Target | |
| Gene ID | 25674 |
| Swiss Prot | P07727 |
| Synonyms | GLRA1 antibody, GLRA1_HUMAN antibody, Glycine receptor 48 kDa subunit antibody, lycine receptor alpha 1 antibody, Glycine receptor strychnine-binding subunit antibody, Glycine receptor subunit alpha-1 antibody, Glycine receptor, alpha 1 subunit antibody, HKPX1 antibody, STHE antibody |
| Background | Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002). |
| Application Dilution | |
| WB | |
| IHC | 1:300-5000 |
Frequently asked questions
Which applications is Glycine Receptor Antibody (bs-70127R) validated for, and at what dilution?
bs-70127R is validated for WB; IHC (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Glycine Receptor Antibody (bs-70127R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-70127R be stored?
The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working Storage buffer: Lyophilized from 5 mM ammonium biocarbonate.
What immunogen was used to raise Glycine Receptor Antibody (bs-70127R)?
Synthetic peptide corresponding to amino acid residues from the N-terminal region of the α1 subunit of rat glycine, conjugated to keyhole limpet hemocyanin (KLH).