Glycogen synthase kinase 3 B (GSK3B) (Ser9) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70128r |
| Product Name | Glycogen synthase kinase 3 B (GSK3B) (Ser9) Antibody |
| Applications |
WB
|
| Specificity | Specific for the ~46 kDa GSK3β protein phosphorylated at Ser9. Also weakly labels the ~51 kDa GSK3β band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Bovine, Chicken, Zebrafish, Xenopus, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat GSK3β, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 84027 |
| Swiss Prot | P18266 |
| Synonyms | Glycogen Synthase Kinase 3 Beta antibody, Glycogen synthase kinase-3 beta antibody, GSK 3 beta antibody, GSK-3 beta antibody, GSK3B antibody, GSK3B_HUMAN antibody, GSK3beta isoform antibody, Serine/threonine-protein kinase GSK3B antibody |
| Background | Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3α and GSK3β. Specifically, GSK3β has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser-9 phosphorylation underlies the inhibition of GSK3β by insulin (Sutherland et al., 1993). |
| Application Dilution | |
| WB | 1:300-5000 |