Interferon-_ Receptor, Type I, Subunit I (Ser535,539) Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70134r |
| Product Name | Interferon-_ Receptor, Type I, Subunit I (Ser535,539) Antibody |
| Applications |
WB
|
| Specificity | Specific for IFNAR1 protein phosphorylated at Ser535,539. Note: the molecular weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110 kDa - 130 kDa. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Bovine, Sheep, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 of human interferon-α, conjugated to keyhole limpet hemocyanin (KLH). |
| Modification Site | Ser535,539 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 3654 |
| Swiss Prot | P17181 |
| Synonyms | Alpha-Type Antiviral Protein, AVP, Beta-Type Antiviral Protein, CRF2-1, Cytokine Receptor Class-II Member 1, Cytokine Receptor Family 2 Member 1, IFN-Alpha/Beta Receptor 1, IFNBR, IFN-Alpha-REC, IFNAR, IFN-R-1, IFRC, Interferon Alpha/Beta Receptor 1, Interferon-Alpha/Beta Receptor Alpha Chain, Interferon Alpha And Beta Receptor Subunit 1, Interferon (Alpha, Beta And Omega) Receptor 1, Interferon-Beta Receptor 1, Interferon Receptor 1, Type I Interferon Receptor 1 |
| Background | Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine-535 and Ser-539 in the IFNAR1 (Kumar et al., 2003). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Interferon-_ Receptor, Type I, Subunit I (Ser535,539) Antibody (bs-70134R) validated for, and at what dilution?
bs-70134R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Interferon-_ Receptor, Type I, Subunit I (Ser535,539) Antibody (bs-70134R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Bovine, Sheep, Non-Human Primate.
How should bs-70134R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Interferon-_ Receptor, Type I, Subunit I (Ser535,539) Antibody (bs-70134R)?
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 of human interferon-α, conjugated to keyhole limpet hemocyanin (KLH).