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SSX5 Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
Overview
Catalog # bs-7013R-APC-Cy7-100uL
Product Name SSX5 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SSX5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6758
Subcellular location Nucleus
Synonyms Protein SSX5; Synovial sarcoma, X breakpoint 5; SSX5_HUMAN.
Background The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This gene appears not to be involved in this type of chromosome translocation. Two transcript variants encoding distinct isoforms have been identified for this gene.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SSX5 Polyclonal Antibody, APC-Cy7 Conjugated (bs-7013R-APC-Cy7) validated for, and at what dilution?

bs-7013R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-7013R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SSX5 Polyclonal Antibody, APC-Cy7 Conjugated (bs-7013R-APC-Cy7)?

KLH conjugated synthetic peptide derived from human SSX5

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-7013R-APC-Cy7)?

APC-Cy7 on bs-7013R-APC-Cy7: excitation / emission 650nm/780nm.