MARCKS Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70144ch |
| Product Name | MARCKS Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~80 kDa MARCKS protein. |
| Reactivity | Human |
| Predicted Reactivity | Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Chicken |
| Source | Recombinant human MARCKS expressed in and purified from E. coli |
| Clonality | Polyclonal |
| Isotype | IgY |
| Concentration | Lot Dependent |
| Purification | Total IgY fraction |
| Storage Buffer | PBS + 10 mM NaN3. |
| Storage Condition | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy |
| Target | |
| Gene ID | 4082 |
| Swiss Prot | P29966 |
| Synonyms | 80 kDa protein antibody, 80K L antibody, 80K L protein antibody, 80K-L protein antibody, 80KL antibody,81 kDa protein, light chain antibody, light chain antibody, MACS antibody, MARCKS antibody, MARCS antibody, MARCS_HUMAN antibody, MGC52672 antibody, myristoylated alanine rich C kinase substrate antibody, Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L) antibody, Myristoylated alanine rich protein kinase C substrate antibody, Myristoylated alanine-rich C-kinase substrate antibody, Phosphomyristin antibody, PKCSL antibody, PRKCSL antibody, protein kinase C substrate 80 kDa protein light chain antibody, Protein kinase C substrate antibody |
| Background | Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentratedin synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al.1995). MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser-152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |
Frequently asked questions
Which applications is MARCKS Antibody (bs-70144CH) validated for, and at what dilution?
bs-70144CH is validated for WB (1:300-5000); IHC. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MARCKS Antibody (bs-70144CH) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Non-Human Primate.
How should bs-70144CH be stored?
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy Storage buffer: PBS + 10 mM NaN3.
What immunogen was used to raise MARCKS Antibody (bs-70144CH)?
Recombinant human MARCKS expressed in and purified from E. coli