MEK1 (Thr386) Antibody

Applications

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Bovine
Chicken
Xenopus
Non-Human Primate

Overview
Catalog #	bs-70154r
Product Name	MEK1 (Thr386) Antibody
Applications	WB
Specificity	Specific for endogenous levels of the ~45 kDa MEK 1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with _-phosphatase.
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Bovine, Chicken, Xenopus, Non-Human Primate
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 of human MEK1, conjugated to keyhole limpet hemocyanin (KLH).
Modification Site	Thr386
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	Lot Dependent
Purification	Antigen Affinity purification from Pooled whole antiserum
Storage Buffer	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 g per ml BSA and 50% glycerol.
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Gene ID	5604
Swiss Prot	Q02750
Synonyms	Dual specificity mitogen activated protein kinase kinase 1 antibody, Dual specificity mitogen-activated protein kinase kinase 1 antibody, ERK activator kinase 1 antibody, MAP kinase kinase 1 antibody, MAP kinase/Erk kinase 1 antibody, MAP2K1 antibody, MAPK/ERK kinase 1 antibody, MAPKK 1 antibody, MAPKK1 antibody, MEK 1 antibody, Mek1 antibody, MEKK1 antibody, Mitogen activated protein kinase kinase 1 antibody, MKK 1 antibody, MKK1 antibody, MP2K1_HUMAN antibody, PRKMK1 antibody, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody, Protein kinase mitogen activated kinase 1 antibody
Background	MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr-292 and Thr-386 (Mansour et al., 1994).
Application Dilution
WB	1:300-5000