Mitochondrial Fission Factor (Mff) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70161r |
| Product Name | Mitochondrial Fission Factor (Mff) Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~38 kDa Mff protein. |
| Reactivity | Human, Mouse, Bovine |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human recombinant protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Neat whole antiserum |
| Storage Buffer | Neat whole antiserum + 0.09% NaN3 |
| Storage Condition | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy |
| Target | |
| Gene ID | 56947 |
| Swiss Prot | Q9GZY8 |
| Synonyms | C2orf33 antibody, Chromosome 2 open reading frame 33 antibody, DKFZp666J168 antibody, GL004 antibody, Mff antibody, MFF_HUMAN antibody, MGC110913 antibody, Mitochondrial fission factor antibody, OTTHUMP00000164235 antibody |
| Background | Mitochondrial fission factor, also known as Mff, is a tail-anchored, outer mitochondrial membrane protein that is part of a complex process controlling mitochondrial and peroxisomal fission in conjunction with Drp1 and Fis1 (Schrader and Yoon, 2007). The rate of mitochondrial fission and fusion balance each other for cell growth and survival of mitochondria. Fission can be greatly accelerated when cytochrome c is released during apoptosis (Desagher and Martinou, 2000). Mff was identified as an important component of the process through siRNA transfected cells, isolating the protein in the P2 pellet and demonstrating that Mff is exposed to the cytosol (Gandre-Babbe and van der Bliek 2008). Mff has been identified at different stages of the fission process working alongside, rather than in complex with, Fis1 suggesting that Mff contributes to fission independent of the Fis1 complex (Gandre-Babbe and van der Bliek 2008). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Mitochondrial Fission Factor (Mff) Antibody (bs-70161R) validated for, and at what dilution?
bs-70161R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mitochondrial Fission Factor (Mff) Antibody (bs-70161R) react with?
Tested reactivity: Human, Mouse, Bovine.
How should bs-70161R be stored?
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy Storage buffer: Neat whole antiserum + 0.09% NaN3
What immunogen was used to raise Mitochondrial Fission Factor (Mff) Antibody (bs-70161R)?
Human recombinant protein