Mnk2a Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-70162r |
| Product Name | Mnk2a Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~52 kDa Mnk2a protein. Immunolabeling is blocked by preadsorption with the Mnk2a peptide used as antigen, but not by Mnk2b peptide. |
| Reactivity | Human |
| Predicted Reactivity | Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Synthetic peptide from the C-terminal region, specific to the human Mnk2a isoform. The sequence of the antigen has no homology with Mnk1 or Mnk2b. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 2872 |
| Swiss Prot | Q9HBH9 |
| Synonyms | ADCAD1 antibody, MADS box transcription enhancer factor 2 polypeptide A (myocyte enhancer factor 2A) antibody, MEF2 antibody, MEF2A antibody, MEF2A_HUMAN antibody, Myocyte enhancer factor 2A antibody, Myocyte-specific enhancer factor 2A antibody, RSRFC4 antibody, RSRFC9 antibody, whole antiserum response factor like protein 1 antibody, whole antiserum response factor-like protein 1 antibody |
| Background | MAP kinase-interacting kinases Mnk1 and Mnk2 are activated by the MAP kinases, ERK and p38 (Waskiewicz et al., 1997). Additionally, Mnk1 and Mnk2 have been shown to phosphorylate the translation initiation factor eIF4E (Fukunaga and Hunter 1997; Waskiewicz et al., 1997). Alternative splicing of the MKNK2 gene results in two isoforms with different C-termini: one that has a MAPK-binding domain, Mnk2a, and one that does not, Mnk2b (Parra et al., 2005). Recent studies have indicated that Mnk2a has tumor suppressive activity while Mnk2b is pro-oncogenic (Maimon et al., 2014). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Mnk2a Antibody (bs-70162R) validated for, and at what dilution?
bs-70162R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mnk2a Antibody (bs-70162R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Non-Human Primate.
How should bs-70162R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Mnk2a Antibody (bs-70162R)?
Synthetic peptide from the C-terminal region, specific to the human Mnk2a isoform. The sequence of the antigen has no homology with Mnk1 or Mnk2b.