Nicotinic Acetylcholine Receptor (nAChR) _4 Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-70178r |
| Product Name | Nicotinic Acetylcholine Receptor (nAChR) _4 Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~52 kDa nAChRβ4 protein. |
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Fusion protein from the cytoplasmic loop of the β4 subunit of mouse nAChR. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Antigen Affinity purification from Pooled whole antiserum |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 108015 |
| Swiss Prot | Q8R493 |
| Synonyms | acetylcholine receptor nicotinic beta 4 (neuronal) antibody, ACHB4_HUMAN antibody, AChR antibody, Cholinergic receptor nicotinic beta 4 antibody, Cholinergic receptor nicotinic beta polypeptide 4 antibody, cholinergic receptor nicotinic beta polypeptide 4 antibody, Chrnb4 antibody, Neuronal acetylcholine receptor subunit beta-4 antibody, Neuronal nicotinic receptor beta 4 subunit antibody |
| Background | Nicotinic acetylcholine receptors (nAChRs) are ionotropic, cholinergic receptors that are divided into 2 types; muscle type and neuronal type. Neuronal nAChRs are pentameric ion channels consisting of 5 identical (homopentamers) or different (heteropentamers) subunits. Heteropentameric neuronal nAChRs mediate fast synaptic transmission in the autonomic nervous system. The predominant hetero-oligomeric nAChR in the CNS contain the subunits α4β2, whereas α3β4 prevail in the PNS. However, the expression of these subunits varies not only by region but also during development (Scholze et al 2011). In the brain, β2-containing receptors greatly outnumber receptors that contain β4 (McGehee & Role, 1995; Albuquerque, et al., 2009), and in most brain regions, targeted deletion of the β2 subunit virtually abolishes [3H]-epibatidine binding and receptor autoradiography (Zoli, et al., 1998) due to the absence of a β subunit required to form functional nAChRs (Champtiaux & Changeux, 2004). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Nicotinic Acetylcholine Receptor (nAChR) _4 Antibody (bs-70178R) validated for, and at what dilution?
bs-70178R is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nicotinic Acetylcholine Receptor (nAChR) _4 Antibody (bs-70178R) react with?
Tested reactivity: Mouse, Rat.
How should bs-70178R be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Nicotinic Acetylcholine Receptor (nAChR) _4 Antibody (bs-70178R)?
Fusion protein from the cytoplasmic loop of the β4 subunit of mouse nAChR.